Signalling

Part:BBa_K858002:Design

Designed by: Sean Kalra   Group: iGEM12_CU_Boulder   (2012-09-07)


LuxR_G2H


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 174
    Illegal PstI site found at 624
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 624
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 174
    Illegal PstI site found at 624
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 174
    Illegal PstI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No extra mutations were necessary to biobrick this part.


Source

This part was synthesized from the directed mutagenesis sequence reported by Leadbetter et al. 2005

References